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Effect of CCA on mRNA stability in skeletal muscle. Total RNA isolated from TA muscle was incubated with cytosolic proteins from CTRL or STIM EDL muscles of animals subjected to CCA for 7 days. After each time point, total RNA was reisolated and PGC-1α, c-myc, and Tfam along with an internal control S12 (n = 4–10) were examined by RT-PCR. * and †, Statistical significance from respective CTRLs and a main effect for all CTRL mRNAs, respectively.
