Fig. 6.

Cer localizes HERG channels to lipid rafts. HERG expressing HEK-293 cells was treated with either Cntl solution (A) or 10 μM Cer (B) for 10 min, and lipid microdomains were isolated by detergent-free sucrose gradient method (highlighted by rectangles). The 12 fractions from top to bottom were probed for HERG and the lipid raft marker caveolin 1 (Cav1) with anti-HERG and anti-Cav1 antibodies. C: lipid raft fractions (fractions 4 and 5) were quantified and expressed as %total protein in the 12 fractions (n = 3). *P < 0.05.