Fig. 8.

The model reproduces the kinetic effects of ceramide on I_HERG. A: the left shift in the V_1/2 and the I_HERG inhibition (11%) induced by Cer model (●) are compared with values from the Cntl model (□). Currents were measured at −40 mV following 5-s steps to the indicated voltage. The smooth lines represent single Boltzmann equation fits to generate V_1/2 = −4.5 and −10.2 mV and slope = 9 and 9 for Cntl and Cer, respectively. B: model reproduces the Cer-induced acceleration of HERG deactivation.