Table 1.

Primers and probes used for bisulfite sequencing and MSP analysis

Sequence primers/ Location	Sequences
hTERT S1/-442 to -219	F 5' GGGTTATTTTATAGTTTAGGT 3'
	R 5' AATCCCCAATCCCTC 3'
hTERT S2/-208 to +104	F 5' GTTTTGTTTTTTTATTTTTTAGTTT 3'
	R 5' CCAACCCTAAAACCCCAAA 3'
hTERT S3/+88 to +348	F 5' TTTGGGGTTTTAGGGTTGG-3'
	R 5' AACCACCAACTCCTTCAAA-3'
hTERT S4/+319 to +566	F 5' GTAGGTGTTTTGTTTGAAGGA-3'
	R 5' TACCAACAAATAAACCAAC-3'
MSP primers/ Location	Sequences*
hTERT M1/-317 to -262	F 5' TAGATTTTCGGGTTCGTTCG 3'
	R 5' TCTATACCCGCGAATCCACT 3'
	P 5' CGACCTAACCCCGACAACGCAACTA 3'
hTERT M2/+288 to +419	F 5' GAGTAGCGTAGGCGATTTAGGGCGT 3'
	R 5' GTCCAACAACGCGAAACCGAA 3'
	P 5' CGCACAACCTCTACAACACTCGAACCACCAACTC 3'
β actin	F 5' TGGTGATGGAGGAGGTTTAGTAAGT 3'
	R 5' AACCAATAAAACCTACTCCTCCCTTAA 3'
	P 5' ACCACCACCCAACACACAATAACAAACACA 3'

*Methylation specific CpGs are printed in bold