Table 1.
Sequence primers/ Location |
Sequences |
---|---|
hTERT S1/-442 to -219 | F 5' GGGTTATTTTATAGTTTAGGT 3' |
R 5' AATCCCCAATCCCTC 3' | |
hTERT S2/-208 to +104 | F 5' GTTTTGTTTTTTTATTTTTTAGTTT 3' |
R 5' CCAACCCTAAAACCCCAAA 3' | |
hTERT S3/+88 to +348 | F 5' TTTGGGGTTTTAGGGTTGG-3' |
R 5' AACCACCAACTCCTTCAAA-3' | |
hTERT S4/+319 to +566 | F 5' GTAGGTGTTTTGTTTGAAGGA-3' |
R 5' TACCAACAAATAAACCAAC-3' | |
MSP primers/ Location |
Sequences* |
hTERT M1/-317 to -262 | F 5' TAGATTTTCGGGTTCGTTCG 3' |
R 5' TCTATACCCGCGAATCCACT 3' | |
P 5' CGACCTAACCCCGACAACGCAACTA 3' | |
hTERT M2/+288 to +419 | F 5' GAGTAGCGTAGGCGATTTAGGGCGT 3' |
R 5' GTCCAACAACGCGAAACCGAA 3' | |
P 5' CGCACAACCTCTACAACACTCGAACCACCAACTC 3' | |
β actin | F 5' TGGTGATGGAGGAGGTTTAGTAAGT 3' |
R 5' AACCAATAAAACCTACTCCTCCCTTAA 3' | |
P 5' ACCACCACCCAACACACAATAACAAACACA 3' |
*Methylation specific CpGs are printed in bold