Figure 1. Kir4.1 and AQP4 expression in mouse brain and isolated astroglial cells.
A, AQP4 immunoblot in wild-type (+/+) and AQP4 null (−/−) mouse hippocampus. β-actin immunoblot shown for same samples. B, (left) Kir4.1 and β-actin immunoblot in brain hippocampus and cortex. (right) Kir4.1 expression normalized to β-actin expression (SE, n=3, differences not significant). C, Immunofluorescence of AQP4 (red) and GFAP (blue). Bar, 10 μm. D, Immunofluorescence of AQP4 (red) and Kir4.1 (green) in isolated cells from mouse hippocampus (nuclei stained blue with DAPI). Bar, 10 μm.