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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Biometals. 2009 Sep 22;23(1):19–30. doi: 10.1007/s10534-009-9263-y

Fig. 2.

Fig. 2

Zinc-mediated disruption of glutathione homeostasis is mediated by the inhibition of glutathione reductase activity. PAECs exposed to ZnCl2 (0–1 mM) were analyzed to determine GSH-to-GSSG ratio (a) and GR enzymatic activity (b). ZnCl2 causes a dose dependent decrease in the GSH-to-GSSG ratio that is correlated with a decrease in GR activity. Confirming the ZnCl2 mediated inhibition of GR, purified bovine GR was inhibited in vitro when exposed to ZnCl2 (0–100 µM, c). Graph represents average enzymatic activity versus control reaction over 5 min. Error bars ±SD (n = 4). * P < 0.05 versus control; † P < 0.05 versus previous dose