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. Author manuscript; available in PMC: 2011 Jul 15.
Published in final edited form as: Cancer Genet Cytogenet. 2010 Jul 15;200(2):184–190. doi: 10.1016/j.cancergencyto.2010.04.011

Figure 1. Human Metnase interacts with and functionally enhances NHEJ in mouse cells.

Figure 1

(A) V5-tagged Metnase or specific point mutants inactivating either the SET (SET−) or Nuclease (Nuclease−) domains were expressed in NIH 3T3 (A) or ES (B) mouse cells. (C) V5-tagged wild-type or mutant Metnase was expressed in mouse ES cells was immunoprecipitated with anti-V5 antibodies (top) or non-specific IgG (middle). These samples and input control were analyzed by western blot with anti-LigIV antibodies. (D) NIH-3T3 or (E) r15 ES were co-transfected with a linearized U6 (hyg) plasmid and vectors expressing wild-type Metnase, SET- or nuclease-defective Metnase, or empty control vector. Two-step NHEJ/integration frequencies are averages (+ SEM) for 3 determinations. * indicates P ≤ 0.0011 (t test).