Table 1. Translocation products.

All sequenced translocation ends are shown. The error free product is shown first for comparison and then products arising in cells with pCAGGS, wild-type Metnase, or Metnase SET-deficient or nuclease-deficient expression vectors are listed. The 14 bp sequences surrounding the translocation junctions are shown for both 5′ and 3′ directions along with the number of deleted bases outside of the AATA I-SceI overhangs. Products arising more than once are shown with a single sequence and with clone numbers listed. Micro-homology mediated repair products are represented by ^*.

`Error-free translocation product:`
`der(17)`	`…ttcaaaaaaaaTAGGGATAA`		`CAGGGTAATgcatgca…`
`sequence`	`…aagttttttttATCCC`		`TATTGTCCCATTAcgtacgt…`
`pCAGGS:`
`18,24,26,31`	`…aaaaaaaaataggg`	`0 del`	`cagggtaatgcatg…`
`1`	`…acagagcaagactc`	`54 del`	`tttgtgatgcaacc…`
`2`	`…gggaggttgcagtg`	`86 del`	`cttggctccgaaat…`
`3,30`	`…caaaaaaaataggg`	`4 del`	`gtaatgcatgcaag…`
`4,8,10,12,14,16,17,19,20,22,27`	`…caaaaaaaataggg`	`5^* del`	`taatgcatgcaagc…`
`5`	`…tccagcctgggtga`	`61 del`	`atgatttttgtgat…`
`6`	`…acagagcaagactc`	`39 del`	`tggctccgaaatga…`
`7`	`…agcaagactccgtc`	`38 del`	`ctccgaaatgattt…`
`9`	`…gacagagcaagact`	`40 del`	`tggctccgaaatga…`
`11,21,25`	`…cgtctcaaaaaaaa`	`5^* del`	`cagggtaatgcatg…`
`13`	`…tctcaaaaaaaata`	`8 del`	`taatgcatgcaagc…`
`15`	`…gcctgggtgacaga`	`31 del`	`ggtaatgcatgcaa…`
`23`	`…ccagcctgggtgac`	`64 del`	`atttttgtgatgca…`
`28`	`…tgacagagcaagac`	`39 del`	`cttggctccgaaat…`
`29`	`…gtctcaaaaaaaat`	`13 del`	`gcatgcaagcttgg…`
`32`	`…ctcaaaaaaaatag`	`2^* del`	`cagggtaatgcatg…`
`Metnase WT:`
`19`	`…aaaaaaaaataggg`	`0 del`	`cagggtaatgcatg…`
`1`	`…aaaaaaaaataggg`	`7^* del`	`atgcatgcaagctt…`
`2`	`…tctcaaaaaaaata`	`3^* del`	`cagggtaatgcatg…`
`3`	`…ctcaaaaaaaatag`	`97 del`	`gaaatgcgagctaa…`
`4`	`…gactccgtctcaaa`	`40 del`	`atgatttttgtgat…`
`5`	`…caaaaaaaataggg`	`4 del`	`gtaatgcatgcaag…`
`6,9,10,11,14–17,20,21`	`…caaaaaaaataggg`	`5^* del`	`taatgcatgcaagc…`
`7`	`…tgacagagcaagac`	`54 del`	`atttttgtgatgca…`
`8`	`…ggtgacagagcaag`	`23 del`	`cagggtaatgcatg…`
`12`	`…aaaaaaaaataggg`	`8^* del`	`tgcatgcaacgttg…`
`13`	`…ctccgtctcaaaaa`	`8^* del`	`cagggtaatgcatg…`
`18`	`…ctcaaaaaaaatag`	`2^* del`	`cagggtaatgcatg…`
`22`	`…acagagcaagactc`	`72 del`	`attttaggggacct…`
`23`	`…agggcctctagtct`	`229^* del`	`agctaagtagacac…`
`24`	`…aaaaaaaaataggg`	`6^* del`	`aatgcatgcaagct…`
`25`	`…caaaaaaaaatagg`	`25 del`	`tccgaaatgatttt…`
`Set Domain Point Mutant:`
`15,20,24,31,33`	`…aaaaaaaaataggg`	`0 del`	`cagggtaatgcatg…`
`1`	`…tctcaaaaaaaata`	`3^* del`	`cagggtaatgcatg…`
`2,4,7–9,13,14,16,19,25,35`	`…caaaaaaaataggg`	`5^* del`	`taatgcatgcaagc…`
`3`	`…caaaaaaaataggg`	`7^* del`	`atgcatgcaagctt…`
`5,21`	`…cgtctcaaaaaaaa`	`5^* del`	`cagggtaatgcatg…`
`6`	`…ccgtctcaaaaaaa`	`58 del`	`attttaggggacct…`
`10`	`…gagctgagatcgcg`	`81 del`	`cgaaatgatttttg…`
`11`	`…gtgagctgagatcg`	`82 del`	`ccgaaatgattttt…`
`12,17,27`	`…tcaaaaaaaatagg`	`47 del`	`aaatgatttttgtg…`
`18`	`…gtgacagagcaaga`	`162 del`	`ctaagtagacacaa…`
`22`	`…caagactccgtctc`	`48 del`	`ttggctccgaaatg…`
`23`	`…aacctgcccaacat`	`205 del`	`catgcaagcttggc…`
`26`	`…acaagggcctctag`	`174 del`	`atgcaaccctattt…`
`28`	`…tgggtgacagagca`	`43 del`	`ttggctccgaaatg…`
`29`	`…ctgcactccagcct`	`61 del`	`tccgaaatgatttt…`
`30`	`…tgggtgacagagca`	`27 del`	`cagggtaatgcatg…`
`32`	`…aaaaaaaaataggg`	`22 del`	`gctccgaaatgatt…`
`34`	`…caaaaaaaaatagg`	`6^* del`	`taatgcatgcaagc…`
`36`	`…aaaaaaaaataggg`	`30^* del`	`atgatttttgtgat…`
`Nuclease Domain Point Mutant:`
`14,18`	`…aaaaaaaaataggg`	`0 del`	`cagggtaatgcatg…`
`1`	`…aaaaaaaaataggg`	`6^* del`	`aatgcatgcaagct…`
`2,5,8–10,13,19,20,23`	`…caaaaaaaataggg`	`5^* del`	`taatgcatgcaagc…`
`3`	`…ccactgcactccag`	`61 del`	`tggctccgaaatga…`
`4`	`…acctgcccaacatg`	`204 del`	`catgcaagcttggc…`
`6,16,21`	`…caaaaaaaataggg`	`7^* del`	`atgcatgcaagctt…`
`7`	`…tggtgacaagggcc`	`54 del`	`gtaatgcatgcaag…`
`11`	`…actccgtctcaaaa`	`11 del`	`gaaatgcgagctaa…`
`12`	`…cgtctcaaaaaaaa`	`5^* del`	`cagggtaatgcatg…`
`15`	`…ctgggtgacagagc`	`39 del`	`gcAAGCTTggctcc…`
`17`	`…ggtgacagagcaag`	`23 del`	`cagggtaatgcatg…`
`22`	`…gagcaagactccgt`	`23 del`	`atgcatgcaagctt…`

Micro-homology mediated repair product