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Overexpression of Pax6a induces neural genes. A, Overexpression of Pax6a, but not Pax6b in hESCs, maintains Sox2 expression. B, C, Pax6a positive cells express pan neural markers, Fabp7 and N-cadherin. D, Most Pax6a-overexpressing cells do not express Sox1. However, some cells with a lower Pax6a level (weaker GFP) are positive for Sox1 (arrows). Scale bars, 50 μm.
