Table 1.
Comparison of intravenous vs. single mosquito bite challenge models
| IV Challenge | Single Mosquito Bite e) | |||||
|---|---|---|---|---|---|---|
| Average | Standard | Success | Average | Standard | Success | |
| Mouse Strains Tested | LD(50) a) | Error | Rate b) | % Infected | Error | Rate |
| BALB/c (Success) | 50c) | 15 | 63% | 98 d) | 1.3 | 80% |
| BALB/c (Failed) | 259 c) | 78 | N/A | 75 d) | 5 | N/A |
| C57BL/6 | 19 c) | 0 | 100% | 100 d) | 0 | 100% |
| ICR (CD-1) | 18 c) | 0 | 100% | 100 d) | 0 | 100% |
a) ID50's were calculated by the Reed-Muench method [46]
b) Success was defined as 90% or better of the naïve animals (challenge controls) being infected
c) Data obtained from 18 (BALB/c) or two (C57BL/6 and ICR (CD-1)) independent IV challenge experiments
d) Data obtained from 11 (BALB/c) or two (C57BL/6 and ICR (CD-1)) independent mosquito bite challenge experiments
e) Mice were challenged by a single infected female mosquito in the presence of two male mosquitoes (as described in Table 2)