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Immunoprecipitations of LRP-1-containing complexes (IP: LRP-1-α) were performed by using anti-LRP-1 α-chain (8G1) antibodies and the immunocomplexes were immunoblotted (IB) by using 8G1, anti-LRP-1 β-chain (5A6), anti-ERK-1/2, anti-phospho-ERK-1/2, anti-JNK-1/2/3, anti-phospho-JNK-1/2/3 and anti-Src antibodies. Nonspecific IgGs were used as a negative control of immunoprecipitation.
