Table 3. Results of topical Juvenile Hormone analogue (JHA) application on larval cuticle brightness (B-value) in the fifth instar and on the length of the fourth instar.

						Avg B-value (±95% CI)					Avg Length of instar (±95% CI) in days
Experiment	Strain	Dose of JHA (µg/µl)	N	DTI (hrs)	N	JHA	N	A ctrl	F	p	JHA	A ctrl	F	p
I: treatment a	dl	0.01	10	0–20	10	26.6±5.2	10	25.9±1.9	0.06	0.81	5.3±0.76	4.6±0.32	0.67	0.42
I: treatment b	dl	1	10	0–20	10	28.6±2.2	10	25.9±1.9	3.39	0.08	5.8±0.76	4.6±0.32	2.94	0.1
I: treatment c	dl	5	10	0–20	10	24.1±2.6	10	25.9±1.9	1.2	0.29	5.5±0.73	4.6±0.32	1.22	0.28
I: treatment d	dl	10	10	0–20	10	25.3±2.9	10	25.9±1.9	0.12	0.74	5.4±0.60	4.6±0.32	1	0.33
II	wt	1	5	0–20	5	57.9±4.9	5	54.4±4.1	0.16	0.7	4.8±0.39	4.7±0.30	1.08	0.31
III	dl	1	5	24–44	5	26.4±2.4	5	25.8±3.6	3.2	0.11	4.8±0.40	5.6±0.80	0.06	0.82
IV	dl	1	5	48–68	5	27.8±6.0	5	24.6±3.3	0.33	0.58	4.4±0.50	4.6±0.50	0.84	0.39
V	dl	1	5	72–92	5	25.6±2.5	5	26.8±7.7	4.5	0.07	4.2±0.40	4.8±0.40	0.08	0.79
VI	dl	1	10	HCA	10	24.5±6.3	10	28.3±3.4	0	1	5.0±0.00	5.0±0.00	3.09	0.1

F statistics represent results from Analysis of Variance to test for significant differences across experimental and control treatments in larval color and development time. Experiment; experiment number. DTI; developmental interval when JHA was applied. A ctrl; Acetone control. N; numbers treated. JHA; experimental groups treated with JHA. HCA; time of head cap apolysis.