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. 2010 Jul 14;5(7):e11575. doi: 10.1371/journal.pone.0011575

Table 6. Synthesis of ribosomes of M. fortuitum and comparison with E. coli B/r.

Ribosomal Component Parameter M. fortuitum * E. coli
μ (h−1) 0.026 0.42
Ribosomes n R(av) (ribosomes) 4,000# 6,800
16S rRNA ε rrs (nucleotides h−1) 47,300±13,920 306,000
n RNAP(rrs)(av) 4.00 17.2
n RNAP(rrs)(av)/n R(av) 1.00×10−3 2.5×10−3
rpsL n c-p(i)/R (copies/ribosome) 1.0 1.0
ε mRNA(i) (nucleotides h−1) 4,575±3,280 129,600
ε aa(i) (amino acids h−1) 1,525±1,096 43,200
n R(i)(av) (ribosomes/cell) 8.52 8.2
n tr(i)(av) (transcripts/cell) 3.00 2.8
rplL n c-p(j)/R (copies/ribosome) 4.0 4.0
ε mRNA(j) (nucleotides h−1) 9,400±2,520 259,200
ε aa(j) (amino acids h−1) 3,150±840 86,400
n R(j)(av) (ribosomes/cell) 17.17 16.0
n tr(j)(av) (transcripts/cell) 5.80 5.8

Comparison of data calculated for M. fortuitum grown in Middlebrook 7H9 with E. coli B/r grown in succinate medium.

*, this study (see Tables 3 and 4).

#

, estimated value (see text).

, data for E. coli B/r [20]. The value for n RNAP(rrs) was calculated using equations (1) and (2): n R(i)(av) and n R(j)(av) were calculated using equations (8) and (9); n tr(i)(av) and n tr(j)(av) were derived from n R(i)(av) and n R(j)(av) by means of n R(i)/tr(i) and n R(j)/tr(j). The value of ε aa(av) was assigned to ε aa(i) as reported for rpsA [23].