Table 6. Synthesis of ribosomes of M. fortuitum and comparison with E. coli B/r.
Ribosomal Component | Parameter | M. fortuitum * | E. coli † |
μ (h−1) | 0.026 | 0.42 | |
Ribosomes | n R(av) (ribosomes) | 4,000# | 6,800 |
16S rRNA | ε rrs (nucleotides h−1) | 47,300±13,920 | 306,000 |
n RNAP(rrs)(av) | 4.00 | 17.2 | |
n RNAP(rrs)(av)/n R(av) | 1.00×10−3 | 2.5×10−3 | |
rpsL | n c-p(i)/R (copies/ribosome) | 1.0 | 1.0 |
ε mRNA(i) (nucleotides h−1) | 4,575±3,280 | 129,600 | |
ε aa(i) (amino acids h−1) | 1,525±1,096 | 43,200 | |
n R(i)(av) (ribosomes/cell) | 8.52 | 8.2 | |
n tr(i)(av) (transcripts/cell) | 3.00 | 2.8 | |
rplL | n c-p(j)/R (copies/ribosome) | 4.0 | 4.0 |
ε mRNA(j) (nucleotides h−1) | 9,400±2,520 | 259,200 | |
ε aa(j) (amino acids h−1) | 3,150±840 | 86,400 | |
n R(j)(av) (ribosomes/cell) | 17.17 | 16.0 | |
n tr(j)(av) (transcripts/cell) | 5.80 | 5.8 |
Comparison of data calculated for M. fortuitum grown in Middlebrook 7H9 with E. coli B/r grown in succinate medium.
, estimated value (see text).
, data for E. coli B/r [20]. The value for n RNAP(rrs) was calculated using equations (1) and (2): n R(i)(av) and n R(j)(av) were calculated using equations (8) and (9); n tr(i)(av) and n tr(j)(av) were derived from n R(i)(av) and n R(j)(av) by means of n R(i)/tr(i) and n R(j)/tr(j). The value of ε aa(av) was assigned to ε aa(i) as reported for rpsA [23].