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. 2010 Jul 14;5(7):e11576. doi: 10.1371/journal.pone.0011576

Figure 5. Induction of HIF-1 activation in HeLa-229 cells by different S. aureus strains defective in the expression of cell wall components.

Figure 5

The HIF-1α protein was analyzed in Western blots six hours upon infection (loading control: actin) of HeLa 229 cells with the following bacterial strains: (A) S. aureus 8325-4 (wt), S. aureus ermBΩhemB 8325-4 (SCV), S. aureus SA113 (wt), S. aureus SA113 Δypf::ermB (reduced in lipoteichoic acid synthesis), S. aureus SA113 ΔtagO (defective in producing wall teichoic acid); (B) S. aureus ATCC 25923 (wt), S. epidermidis ATCC 12228 (wt), S. aureus Newman (wt), S. aureus Newman mAH12 (defective in producing the extracellular adherence protein EAP). Negative control: uninfected cells, positive control: DFO (200 µM). Multiplicity of infection (MOI): 20.