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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Traffic. 2010 May 26;11(8):1067–1078. doi: 10.1111/j.1600-0854.2010.01082.x

Figure 4. Exogenous Golgi membranes bind permeabilized cells.

Figure 4

(A) NRK cells were mock treated or permeabilized by a freeze/thaw cycle as indicated. Rat-liver Golgi particles were incubated with cytosol, GTPγS, and an ATP-regenerating system then labeled with bodipy-C5-ceramide (red), and added to the permeabilized cells at 37°C. The nuclei were labeled using DRAQ5 (blue). In two independent experiments, no exogenous membranes were found bound to intact cells (n=20 cells) while an average of 0.6 membrane particles was observed per permeabilized cell (n=19 cells). The bar represents 10 μm. (B) Permeabilized Vero cells were incubated with rat-liver Golgi membranes (red). The cells were fixed and the endogenous Golgi membranes were decorated with a primate-specific antibody against giantin (green). The nuclei were labeled using DRAQ5 (blue).