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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Traffic. 2010 May 26;11(8):1067–1078. doi: 10.1111/j.1600-0854.2010.01082.x

Figure 5. Exogenous Golgi membranes undergo microtubule-dependent entry into permeabilized cells.

Figure 5

(A) Shown are confocal micrographs of NRK cells that were fixed at the indicated time points relative to permeabilization and incubation. The cells were decorated with antibodies against the Golgi marker GM130 (green) and microtubules (red). The nuclei were labeled using DRAQ5 (blue). (B) Shown are confocal micrographs indicating actin distribution at the indicated time points relative to the permeabilization and incubation. (C) NRK cells were either mock treated (control) or incubated with 20 μM nocodazole before and after permeabilization. Rat-liver Golgi membranes (red) and cytosol were added to the cells, and cell-associated membranes were visualized by confocal microscopy. The bar represents 20 μm. (D) NRK cells were treated as in (C) and the average number of Golgi particles per cell was determined from four independent experiments by blind counting with (n=85 cells) or without (n=111 cells) nocodazole treatment. The standard error is indicated by bars.