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. Author manuscript; available in PMC: 2011 Aug 15.
Published in final edited form as: Arch Biochem Biophys. 2010 Jun 8;500(2):144–150. doi: 10.1016/j.abb.2010.06.001

Fig. 2. Binding of N-terminal truncated TnT fragments to Tm.

Fig. 2

N-terminal serially truncated cardiac TnT fragments (Fig. 1) were tested for Tm-binding affinity in ELISA protein binding experiments. The results showed that the deletion of the N-terminal variable region (McTnT-ND72) alone did not significantly affect Tm-binding whereas further deletion of 20 amino acids in the conserved middle region (McTnT-ND92) produced a detectable decrease in Tm-binding affinity. Extending the deletion to remove 39 more amino acids encoded by exon 10 (McTnT-ND130) resulted in a major decrease in Tm-binding affinity.