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. Author manuscript; available in PMC: 2011 Aug 15.
Published in final edited form as: Arch Biochem Biophys. 2010 Jun 8;500(2):144–150. doi: 10.1016/j.abb.2010.06.001

Fig. 3. mAb competition/blocking mapping of the T1 region Tm-binding site of TnT.

Fig. 3

(A) The competition curves showed that mAb 2C8 significantly blocked the binding of TnT to Tm (P<0.01). In contrast, mAbs CT3 and T12 recognizing the flanking regions (Fig. 1) had only marginal effects. (B) The ELISA titration curves showed that mAb 2C8 raised against mouse cardiac TnT binds fast, slow and cardiac TnT isoforms with very similar affinities, indicating a highly conserved epitope structure with conserved function. Data are plotted as mean ± SD.