Table 3. Comparison of z and V_0.5 values obtained from fitting experimental and simulated data.

Ionic conditions are indicated in the left column. Statistical differences were evaluated using one way ANOVA with p<0.5. z values were statistical different when [Cl^-] decreased from 140 or 70 to 25 mM but not when decreased from 140 to 70 mM. Also z values were different when the pH was varied between 5.5, 7.3 and 9. V_0.5 values were not different when [Cl^-] was decreased from 140 or 70 to 25 mM but were different by decreasing [Cl^-] from 140 to 70 mM. No statistical differences were found in V_0.5 when pH was decreased to 5.5 or increased to 9. In contrast V_0.5 values were different when compared pH 9 and 5.5. Values given are mean±SDM.

	Experiment		Simulation
Ionic condition	z	V0.5 (mV)	z	V0.5 (mV)
[Cl]i=140, [Cl]e=140, pH=7.3	1.34±0.09	80.8±8.1	1.26	66.9
[Cl]i=70, [Cl]e=140, pH=7.3	1.21±0.14	100.0±10.8	1.24	68.9
[Cl]i=25, [Cl]e=140, pH=7.3	0.76± 0.29	89.8±12.3	1.22	71.5
pH=5.5, [Cl]i=[Cl]e=140	1.33±0.11	63.5±8.	1.37	56.6
pH=7.3, [Cl]i=[Cl]e=140	1.17±0.11	79.3±11.4	1.26	66.9
pH=9, [Cl]i=[Cl]e=140	0.95±0.42	85.1±17.0	1.21	73.3