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. Author manuscript; available in PMC: 2010 Jul 15.
Published in final edited form as: Mol Cell. 2006 Apr 21;22(2):259–268. doi: 10.1016/j.molcel.2006.03.030

Table 1.

Measured Km values of C9Holo, t-C9Holo, LZ-C9 and t-LZ-C9 for LEHD-AFC and pro-caspase-3.

Km for LEHD-AFC Km for pro-caspase-3
C9Holo 686.2 ± 136.9 μM† Average 139.3 ± 23.19 nM#, &

  1. 155.7 ± 86.2 nM†

  2. 122.9 ± 28.1 nM†
t-C9Holo Average 276.5 ± 65.48 μM#, $

  1. 230.2 ± 76.2 μM†

  2. 322.8 ± 250.2 μM†

 79.8 ± 18.5 nM†
LZ-C9 Average 465.7 ± 118.4 μM#, &

  1. 382 ± 185.1 μM†

  2. 549.4 ± 231.8 μM†

 High@
t-LZ-C9 Average 260.0 ± 22.84 μM#, $

  1. 243.8 ± 194.7 μM†

  2. 276.1 ± 240.3 μM†

 High@
†

The errors in individual Km measurements represent the scatter of the data points from the hyperbolic fittings.

#

When two measurements are available, the average Km values are shown along with the individual measurements. The errors in average Km values are standard deviations from the two measurements.

$

One protein preparation was used in the two individual measurements for obtaining the average Km values.

&

Two different protein preparations were used in the two individual measurements for obtaining the average Km values.

@

Km values could not be determined from the current data.