Table 2.
Importance of IL-12-IFN-γ axis for in vivo LPS sensitization by P. acnes treatment. Mice with various genotypes were sequentially administered with P. acnes and LPS. At day 7 after P. acnes priming, hepatic granuloma formation was determined by histological analyses.
| P. acnes-primed mice | Sensitization phase | LPS phase | ||||
|---|---|---|---|---|---|---|
| Granuloma formationa | Hypothermiab | Hypercoagulationc | Serum TNF-α d | Lethalitye | Liver injuryf | |
| WT | +++ | +++ | +++ | +++ | 100 | +++ |
| I l12p40−/− | − | − | − | − | 0 | − |
| I f n γ −/− | − | − | − | − | 0 | − |
a +++ indicates that 20% and more area of the liver section is occupied by granulomas; − indicates no granulomas.
b +++ indicates more than 5°C reduction of rectal temperature after LPS challenge; − indicates less than 1°C of it.
c +++ indicates more than 10 μg/mL of plasma TAT levels; − indicates normal range of them (< 50 ng/mL); ± indicates < 50 ng/mL and > 200 ng/mL.
d +++ indicates more than 5 ng/mL; − indicates less than 0.1 ng/mL.
e Mice were monitored for 48 h after LPS challenge.
f +++ indicates more than 300 IU of serum ALT levels; − indicates normal range of them (< 50 IU).