Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Apr 29;76(5):1095–1110. doi: 10.1111/j.1365-2958.2010.07145.x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2010 Blackwell Publishing Ltd

Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.

PMC Copyright notice

Fig. 1 — BFP expression is reduced in EPEC mutants lacking dsbA, degP and cpxP. A. Western analysis of bundlin (BfpA), BfpB, BfpC and BfpD expression in wild-type and mutant EPEC strains: lane 1, wild-type (E2348/69); lane 2, bfp^- strain (JPN15); lane 3, degP::kan (ALN188); lane 4, ppiA::kan (ALN190); lane 5, cpxP::kan (ALN194); lane 6, dsbA::kan (TR1121). Whole-cell lysates were collected from EPEC grown in DMEM/F12 as described in Experimental procedures. Samples were collected from each strain at least three times; one representative blot is shown. Arrows denote non-specific bands (NSB). B. Results of autoaggregation assay performed on EPEC Cpx regulon mutants. Autoaggregation assays were performed as described in Experimental procedures; the overall average and standard deviation resulting from two separate experiments performed in triplicate are shown. One asterisk (*) denotes a value significantly different from positive control E2348/69; two asterisks (**) denote a value significantly different from both E2348/69 and negative control JPN15 (one-way anova with Bonferroni's multiple comparison test; P < 0.05).