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. 2010 Apr 29;76(5):1095–1110. doi: 10.1111/j.1365-2958.2010.07145.x

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© 2010 Blackwell Publishing Ltd

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Fig. 2 — Overexpression of dsbA in an EPEC cpxR mutant does not restore BFP synthesis. Western blotting was used to detect expression of the proteins bundlin (BfpA) and BfpB in the wild-type strain E2348/69 (ptrc99A) (lane 1), the cpxR mutant ALN88 (ptrc99A) (lane 2), ALN88 (pDsbA) (lane 3), the dsbA mutant TR1121 (ptrc99A) (lane 4) and TR1121 (pDsbA) (lane 5). Whole-cell lysates were collected from EPEC grown in DMEM/F12 without IPTG as described in Experimental procedures. Samples were collected from each strain at least three times; one representative blot is shown.