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. 2010 Apr 29;76(5):1095–1110. doi: 10.1111/j.1365-2958.2010.07145.x

Fig. 4.

Fig. 4

BFP elaboration is inhibited by the cpxA24 gain-of-function mutation. A. Western analysis of bundlin (BfpA), BfpB, BfpC and BfpD expression in wild-type strain E2348/69 (lane 1) and cpxA24 mutant EPEC strain ALN195 (lane 2). Whole-cell lysates were collected from EPEC grown in DMEM/F12 as described in Experimental procedures. Samples were collected from each strain at least three times; one representative blot is shown. Arrows denote non-specific bands (NSB). B. Results of autoaggregation assay performed on wild-type (lane 1) and cpxA24 mutant (lane 2) EPEC. Autoaggregation assays were performed as described in Experimental procedures; the overall average and standard deviation resulting from two separate experiments performed in triplicate are shown. The asterisk (*) denotes a value significantly different from positive control E2348/69 (P < 0.0001, unpaired t-test).