Fig. 5.

BFP expression is reduced when the Cpx response is activated by overexpressing NlpE. Western analysis of bundlin (BfpA), BfpB, BfpC and BfpD expression in wild-type strain E2348/69 harbouring the vector control pCA24N (lane 1) or the overexpression plasmid pCA-nlpE (lane 2). Whole-cell lysates were collected from EPEC grown in DMEM/F12 as described in Experimental procedures. Subcultures were grown for 2 h before being induced with 1 mM IPTG, followed by an additional 3 h incubation at 37°C. Samples were collected from each strain at least three times; one representative blot is shown. Arrows denote non-specific bands (NSB).