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. 2010 Apr 29;76(5):1095–1110. doi: 10.1111/j.1365-2958.2010.07145.x

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© 2010 Blackwell Publishing Ltd

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Fig. 7 — The cpxA24 mutation does not affect BFP elaboration when the bfp genes are expressed from an inducible promoter. A. Western analysis of bundlin (BfpA), BfpB and CpxR expression in the negative control JPN15 (ptrc99A) (lane 1), the positive control JPN15 (pKDS302), which expresses the bfpA-L operon (lane 2), and its isogenic cpxA24 mutant SV76 (lane 3). Whole-cell lysates were collected from EPEC grown in LB containing 1 mM IPTG as described in Experimental procedures. Samples were collected from each strain at least two times; one representative blot is shown. Arrows denote non-specific bands (NSB). B. Results of autoaggregation assay performed on JPN15 (ptrc99A) (lane 1), JPN15 (pKDS302) (lane 2) and SV76 (lane 3). Autoaggregation assays were performed as described in Experimental procedures; the overall average and standard deviation resulting from three separate experiments performed in triplicate are shown.