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. 2010 Apr 29;76(5):1095–1110. doi: 10.1111/j.1365-2958.2010.07145.x

Fig. 8.

Fig. 8

Overexpression of perA in an EPEC cpxA24 mutant restores BFP synthesis. Western blotting was used to detect expression of the proteins bundlin (BfpA) and BfpB in the wild-type strain E2348/69 (lane 1), the cpxA24 mutant ALN195 (lane 2), the vector control (VC) strain ALN195 (pMPM-K3) (lane 3) and the perA-overexpressing strain ALN195 (pCS-A) (lane 4). Whole-cell lysates were collected from EPEC grown in DMEM/F12 with 50 µM IPTG as described in Experimental procedures. Samples were collected from each strain at least twice; one representative blot is shown. The arrow denotes a non-specific band (NSB).