Table 1.
Bacterial strains and plasmids used in this study.
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Bacterial strains | ||
| E2348/69 | Prototypical EPEC O127:H6 strain | Levine et al. (1978) |
| JPN15 | Spontaneous pEAF-cured derivative of E2348/69 | Jerse et al. (1990) |
| ALN88 | E2348/69 cpxR::KanR | Nevesinjac and Raivio (2005) |
| ALN188 | E2348/69 degP::KanR | D.M. MacRitchie et al. (in preparation) |
| ALN190 | E2348/69 ppiA::KanR | D.M. MacRitchie et al. (in preparation) |
| ALN194 | E2348/69 cpxP::KanR | D.M. MacRitchie et al. (in preparation |
| ALN195 | E2348/69 cpxA24 (AmkR) | MacRitchie et al. (2008b) |
| ALN234 | E2348/69 cpxR::CamR | MacRitchie et al. (2008b) |
| TR1121 | E2348/69 dsbA::KanR | D.M. MacRitchie et al. (in preparation) |
| SV76 | JPN15 cpxA24 (pKDS302) (AmkR AmpR) | This study |
| UMD916 | E2348/69 bfpF::KanR | Anantha et al. (1998) |
| SV75 | UMD916 cpxA24 (KanR AmkR) | This study |
| SV82 | UMD916 cpxR::CamR (KanR) | This study |
| Plasmids | ||
| ptrc99A | High copy-number expression vector with IPTG-inducible promoter (AmpR) | Pharmacia |
| pDsbA | ptrc99A-based dsbA overexpression vector (AmpR) | Buelow and Raivio (2005) |
| pJW22 | perA promoter cloned into luxCDABE reporter vector pJW15 (KanR) | MacRitchie et al. (2008b); this study |
| pJW23 | bfpA promoter cloned into luxCDABE reporter vector pJW15 (KanR) | MacRitchie et al. (2008b); this study |
| pCA24N | High copy-number expression vector with IPTG-inducible promoter (CamR) | Kitagawa et al. (2005) |
| pCA-nlpE | pCA24N-based nlpE overexpression vector (CamR) | Kitagawa et al. (2005) |
| pKDS302 | ptrc99A containing the bfpA-L gene cluster expressed from an IPTG-inducible promoter (AmpR) | Stone et al. (1996) |
| pMPM-K3 | Low copy-number cloning vector derived from pACYC184 and pBluescript (KanR) | Mayer (1995) |
| pCS-A | pMPM-K3-derived perA overexpression plasmid (KanR) | Martínez-Laguna et al. (1999) |