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. 2010 Apr 15;76(6):1461–1482. doi: 10.1111/j.1365-2958.2010.07151.x

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© 2010 Blackwell Publishing Ltd

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Fig. 3 — Localization of TbnSMase-HA^Ti in bloodstream form T. brucei. A. Fixed TbnSMase-HA^Ti cells were incubated with rat anti-HA antibody, and rabbit anti-rat FITC conjugated antibody and DNA stained with DAPI. B. Anti-HA Western analysis of fractions from differential centrifugation, 14 500 g pellet (1), 140 000 g pellet (2) and supernatant of 140 000 g pellet (3) of TbnSMase-HA^Ti cells, prepared as described in the Experimental procedures.