Fig. 5.

Mass spectrometric analyses of phospholipids. Choline-containing phospholipids extracted from the P100 fraction as described in Experimental procedures were analysed by ESI-MS/MS in positive ion mode using parent-ion scanning of the collision induced fragment for phosphorylcholine at 184 m/z in (A) parental cells. (B) TbnSMase cKO cells grown in the absence of tetracycline for 42 h. ESI-MS negative ion survey scan spectra of P100 lipid extracts from T. brucei bloodstream form cells. (C) Parental cells. (D) TbnSMase cKO cells grown in the absence of tetracycline for 42 h. Asterisks highlight ions, which are altered significantly in intensity, as described in the text