Table 1.

Sequences for primers used in real-time RT-PCR

Gene	Forward (5′ to 3′)	Reverse (5′ to 3′)
Osteocalcin (m)	CTCTCTCTGCTCACTCTGCT	TTTGTAGGCGGTCTTCAAGC
Type 1 collagen (m)	GCCTCCCAGAACATCACCTA	AGTTCCGGTGTGACTCGTG
Alkaline phosphatase (m)	ATGGTAACGGGCCTGGCTACA	AGTTCTGCTCATGGACGCCGT
IGF-I (m)	CATCTCCAGTCTCCTCAGATC	GTCCACACACGAACTGAAGAGC
IGF-II (m)	AGCGGCCTCCTTACCCAA	CGCGGTCCGAACAGACAA
IGFBP-3 (m)	CCAGAACTTCTCCTCCGAGTCTAAG	CTCAGCACATTGAGGAACTTCAGAT
IGFBP-5 (m)	ATACAACCCAGAACGCCAGCT	ACCTGGGCTATGCACTTGATG
IGFBP-5 (h)	GAGGCAAGTTGGATGAACAG	TGTCCATACTGCTGGCTAGA
FHL-2 (m)	AAAGCCTATCACCACAGGAGGT	CCCAGCACATTTCTTAGCGTAC
FHL-2 (h)	CTTCACAGCTCGCGATGACT	CAAGCAGCAACTTCTCTGTG
ADAM-9 (m)	CCAGACCCAGGGATGGTGAAT	GGCCATGACATTTTCCCTGAA
ADAM-9 (h)	GCATTAACCTCACCGATGAC	GGAGTGTTCCTCGACATGTT
PPIA (m)	TCCTGGACCCAAAACGCTCC	CCATGGCAAATGCTGGACCA
Actin (h)	GAGGCATTGCTGACAGGATG	TGCTGATCCACATCTGCTGG

h = primer specific for human; m = primer specific for mouse; IGF = insulin-like growth factor; IGFBP = IGF binding protein; FHL = four and a half lim; ADAM = a disintegrin and metalloprotease.