Histological analysis of the growth plate and mid-diaphysis of femur. Femur sections of line 26 progeny were subjected to Goldner staining. A, The growth plate (indicated by arrows) was wider in transgenic (Tg) mice compared with wild-type (Wt) littermates at 6 months of age; B, transgenic mice show increased inner and outer diameters in the mid-diaphysis of the femurs at 6 wk of age; C, size-related bone parameters were significantly increased in transgenic mice compared with the wild-type littermates at the age of 6 wk measured by histological analysis (n = 3, mean ± SEM). B. Area, Cortical bone area; Ec. Pm, endocoritcal perimeter; Me. Area, medullary area (bone marrow cavity area); Ps. Pm, periosteal perimeter; Tt.Area, total area (bone marrow cavity area plus cortical bone area). *, P < 0.05; **, P < 0.01; ***, P < 0.001.