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(A) Flow chamber used in our single-molecule experiments with which total internal reflection microscopy can be carried out conveniently. A small sample reservoir shown in yellow and a piece of tubing connected to a syringe are attached to the inlet and outlet of the channel, respectively, to facilitate sample flow. The tubing and syringe are shown for one channel only for clarity. (B) Structure of the ratiometric, dual-emission probe 5-(and-6)-carboxy SNARF-1.
