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. Author manuscript; available in PMC: 2010 Jul 15.
Published in final edited form as: Biochemistry. 2006 Jul 25;45(29):8699–8711. doi: 10.1021/bi060580y

Figure 2.

Figure 2

Summary of the effects of modifications on receptor-stimulated CheA kinase activity in the reconstituted signaling complex. (A) Effect of cysteine modification on receptor-stimulated CheA activity relative to that of Cysless CheA. Values below the indicated threshold are operationally defined as perturbed. (B) Effect of 5-fluorescein-maleimide (5FM) attachment on receptor-stimulated CheA activity relative to that of Cysless CheA. (C) PICM parameter (see text) for each cysteine position. Again, values below the indicated threshold are operationally defined as perturbed. (In panels A and C, five above-threshold modifications were ambiguous because their error bars crossed the threshold: H401C, T436C, R332F1, A412F1, M469F1. All five were found to be nonperturbing in the pull-down assay for core complex binding (56), confirming their identification as nonperturbing modifications and validating the indicated thresholds.)