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Summary of the effects of 5-fluorescein-maleimide (5FM) attachment on CheA binding to its docking partners. (A) Effects of 5FM attachment on relative incorporation of CheA into the core complex as defined by the binding parameter BC (see text, eq 1). The horizontal line indicates operational threshold of strong complex affinity. (B) Effects of 5FM attachment on free CheA binding to CheW. Values shown are the binding affinity (KA = 1/KD), relative to that of Cysless CheA.
