Spatial mapping of results, and working models for core complex architecture. (A) Experimental PICM parameters. Nonperturbing positions (white) lie on surfaces with no detected docking interactions. Perturbing positions assigned to the P1–P4 interaction faces (blue), to the CheW docking site (red), and to an essential surface proposed to bind the receptor (black) are also indicated. (B) Conserved surface residues, where the intensity of red is proportional to the degree of conservation (see Supporting Information). (C and D) Top views of panels A and B, respectively. (E and F) Two working models for core complex architecture. P1, CheW, and receptor are shown in light green, blue, and tan, respectively.