. Author manuscript; available in PMC: 2010 Jul 15.

Published in final edited form as: Biopolymers. 2008;90(3):307–319. doi: 10.1002/bip.20856

Table I.

Signal Peptides Used for Crosslinking to SRP

Peptide	Sequence^a
BioPhoA^b	Biotin-MKQKKKLALLLLLLLLTPVTKA-NH₂
BioPhoA C₂	Biotin-MCKQKKKLALLLLLLLLTPVTKA-NH₂
BioPhoA C₁₀	Biotin-MKQKKKLALCLLLLLLTPVTKA-NH₂
BioPhoA C₁₁	Biotin-MKQKKKLALLCLLLLLTPVTKA-NH₂
BioPhoA C₁₂	Biotin-MKQKKKLALLLCLLLLTPVTKA-NH₂
BioPhoA C₁₃	Biotin-MKQKKKLALLLLCLLLTPVTKA-NH₂
BioPhoA C₁₄	Biotin-MKQKKKLALLLLLCLLTPVTKA-NH₂
BioPhoA C₂₃	Biotin-MKQKKKLALLLLLLLLTPVTKACNH₂
BioPhoA₁₀-Bpa	Biotin-MKQKKKLAL(Bpa)LLLLLLTPVTKA-NH₂
BioPhoA₁₁-Bpa	Biotin-MKQKKKLALL(Bpa)LLLLLTPVTKA-NH₂
BioPhoA₁₂-Bpa	Biotin-MKQKKKLALLL(Bpa)LLLLTPVTKA-NH₂
BioPhoA₁₃-Bpa	Biotin-MKQKKKLALLLL(Bpa)LLLTPVTKA-NH₂
BioPhoA₁₄-Bpa	Biotin-MKQKKKLALLLLL(Bpa)LLTPVTKA-NH₂

Sequences of the PhoA-derived signal peptides used for crosslinking. The positions indicated in bold were replaced by Cys to create the BioPhoAC_n series of peptides or Bpa to create the BioPhoABpa_n series. The BioPhoAC_n-BPM series of peptides was created with the BioPhoAC_n set of peptides by alkylation of the free Cys with 4-maleimido-benzophenone.

Biotinylated wild-type alkaline phospatase (PhoA) signal peptide.