Table 2.
Summary of AML cell-line analysis and MDM2 inhibitor treatment IC50 values
| AML cell line | FAB type | p53 (exon 5-9) status | Immunoblot (MI-219 10μM 8 hours/16 hours) | IC50/MI-219, μM | IC50/MI-63, μM | IC50/Nutlin, μM |
|---|---|---|---|---|---|---|
| SKM-1 | sM5 | mut | Noninducible | > 20 | > 10 | > 10 |
| OCI-M2 | sM6 | mut | Noninducible | > 20 | > 10 | > 10 |
| MONO-MAC-6 | sM5 | mut | Noninducible | > 20 | > 10 | > 10 |
| GF-D8 | M1 | mut | Noninducible | > 20 | > 10 | > 10 |
| MOLM-16 | M0 | mut | Noninducible | > 20 | > 10 | > 10 |
| Kasumi-3 | M0 | mut | Noninducible | > 20 | > 10 | > 10 |
| Kasumi-1 | M2 | mut | Noninducible | > 20 | > 10 | > 10 |
| ME-1 | M4eo | mut | Null | > 20 | > 10 | > 10 |
| AML-193 | M5 | mut | Null | > 20 | > 10 | > 10 |
| KG-1 | M6 | mut | Null | > 20 | > 10 | > 10 |
| HL60 | M2 | deleted | Null | > 20 | > 10 | > 10 |
| OCI-AML-3 | M4 | wt | Inducible | 18.176 | > 10 | > 10 |
| OCI-AML-5 | M4 | wt | Inducible | 13.846 | > 10 | > 10 |
| ML-2 | sM4 | wt | Inducible | 7.038 | 7.468 | 10.447 |
| OCI-AML-2 | M4 | wt | Inducible | 5.248 | 4.735 | 7.216 |
| AP-1060 | M3 | wt | Inducible | 4.224 | 4.029 | 6.768 |
| SIG-M5 | M5a | wt | Inducible | 3.436 | 3.801 | 4.727 |
| MOLM-13 | sM5a | wt | Inducible | 2.657 | 2.855 | 4.044 |
| MUTZ-2 | M2 | wt | Inducible | 1.283 | 1.17 | 0.951 |
mut indicates mutated; and wt, wild-type.