Table 3.

Macrophage Contamination Does Not Explain the Changes Observed With Aging in Preadipocyte Cultures

Macrophage Marker	Young Epididymal	Old Epididymal	Young Perirenal	Old Perirenal	Min. Cycles	Age ANOVA p Value	Age × Depot ANOVA p Value
Cd 68	9.9 ± 2.2	15.9 ± 2.2	14.5 ± 3.7	15.6 ± 3.7	24	0.24	0.41
Ccl3	14.5 ± 6.1	21.0 ± 4.5	11.3 ± 3.5	13.6 ± 4.0	27	0.35	0.65
Cd 11b; Mip-1; α-M integrin	9.2 ± 1.7	13.5 ± 1.8	13.9 ± 3.4	20.1 ± 3.5	25	0.06	0.72
F4/80; Emr1	16.7 ± 4.2	20.3 ± 3.9	11.0 ± 3.9	10.1 ± 3.1	27	0.40	0.25
Scya4; Mip-1β	79.7 ± 7.0	90.6 ± 22.2	205.8 ± 22.1	152.8 ± 9.5	From array	0.09	0.24

Notes: Consistent differences in macrophage markers were not evident among preadipocyte cultures from different age groups. mRNA levels were assayed in primary preadipocyte cultures from perirenal and epididymal depots of young (3 mo) and old (30 mo) rats. Scya4 was the only transcript that met the criterion for being considered detectable on Affymetrix U230 arrays. The other transcripts were assayed by real-time polymerase chain reaction (PCR; N = 6 determinations, each from different sets of rats; means ± SEM are shown; Min. cycles = minimum number of PCR cycles required for detection). ANOVA = analysis of variance.