. Author manuscript; available in PMC: 2011 Mar 1.

Published in final edited form as: J Reprod Immunol. 2010 Jan 20;84(2):145–153. doi: 10.1016/j.jri.2009.11.003

Table 2.

Quantification of adhesion and intercellular/intracellular localization of C. rectus and C. jejuni in trophoblast cells in vitro. Data represent the mean number of bacterial cells counted in the entire stack of images in up to 8 random fields per sample (Fig. 1). C. jejuni and C rectus adhered to SM9-1 murine trophoblast cells but invasion was negligible. In contrast, both C. jejuni and C. rectus invaded the human trophoblast cell line BeWo and the canine epithelial cell MDCK (P < 0.05). It was noted that in most cases C. jejuni adhesion/invasion of cells was significantly greater than that of C. rectus. However, C. rectus showed a non-significant trend of higher invasiveness counts in BeWo cells when compared to C. jejuni (P = 0.08).

Cell line	Bacteria	Bacteria adhered/cell	Bacteria colocalizing with actin (cell junctions) per cell	Invasive bacteria/cell (cytoplasmic)
SM9-1	C. rectus 314	0.50±0.41^*	0.44±0.10^*	0.00±0.00
SM9-1	C. jejuni 43457	4.79±2.82^*	1.53±0.71^*	0.00±0.00
BEWO	C. rectus 314	0.43±0.52^*	0.08±0.11^**	5.51±3.18
BEWO	C. jejuni 43457	5.30±1.75^*	2.83±0.32^**	2.96±0.77
MDCK	C. rectus 314	0.26±0.36^*	0.10±0.12^*	0.69±0.25^*
MDCK	C. jejuni 43457	3.83±2.40^*	1.40±0.65^*	1.84±1.19^*

P < 0.05

^**

P < 0.01.