Table 3.

Relative proinflammatory gene expression in trophoblastic cultures after C. rectus infection in vitro. Data represent mean fold changes±SD normalized to non-infected controls. Cells were infected at MOI 50, 100 and 500 during 12 h and the relative expression of human or murine IL-6 and TNFα was quantified by qPCR.

Gene	MOI	mRNA expression fold differences
Human IL6	50	2.75 ± 0.38
	100	4.18 ± 0.85*
	500	6.39 ± 0.61**
Human TNFα	50	10.9 ± 0.54**
	100	24.6 ± 0.55**
	500	56.4 ± 1.96**
Murine IL6	50	0.5 ± 6.88
	100	4.15 ± 1.87
	500	2.31 ± 2.10
Murine TNFα	50	1.23 ± 0.64
	100	1.0 ± 0.34
	500	1.24 ± 0.49

^*P < 0.05 when compared to non-infected controls.

^**P < 0.001 when compared to non-infected controls.