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Western blot analysis showing quantitative measurement of α-SMA (myofibroblast marker) in ECF treated with or without TGFβ1 (1ng/ml) and 0.02% MMC. Equal quantity of protein (50 μg) was loaded in each lane. GAPDH was used as house keeping gene. A single early MMC treatment showed significant decrease in TGFβ1-induced myofibroblast formation in ECF compared to untreated controls. The late MMC treatment showed moderate reduction in α-SMA.
