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. 2010 Mar 3;87(3):578–584. doi: 10.1093/cvr/cvq059

Figure 2.

Figure 2

Ang1-7 effects on astrocytes imaged within the RVLM in organotypic cultured slices of rat medulla. (A) Ang1-7 (200–1000 nM) causes a dose-dependent activation of astrocytes in RVLM in WR (grey bars) and SHR (black bars). Statistics are based on the fraction of activated astrocytes from the total number of recorded cells as indicated on the top of the bars. (B) Example of an Ang1-7-induced Ca2+ response in astrocytes. Ang1-7 induced fairly slow [Ca2+]i elevations which in some cells could last for a long time even after the drug was discontinued. (C) Pharmacological analysis of Ang1-7 effect. The concentration of 600 nM was used in all these experiments. Note that Ang1-7 effect was blocked by a competitive blocker of the Mas receptor A779 but not losartan, the Ang II antagonist. Two blockers of intracellular stores, CPA, and 2-APB significantly (P < 0.05) attenuated the effect of Ang1-7. The right bar represents the effect of 600 nM Ang1-7 alone for comparison.