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. 2010 Mar 3;87(3):524–534. doi: 10.1093/cvr/cvq068

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Published on behalf of the European Society of Cardiology. All rights reserved. © The Author 2010. For permissions please email: journals.permissions@oxfordjournals.org.

PMC Copyright notice

Generation of double-transgenic mice. (A) Breeding strategy showing generation of p53^−/−/myo^+/+ double-Tg mice in filial generation from Tg mice overexpressing myotrophin in the heart (myo-Tg, i.e. myo^+/+) and p53-null (p53^−/−) parents; (B) Genotyping of filial offspring by using specific sets of primers (as mentioned in the text) to identify p53^−/−/myo^+/+ (no. 440), p53^+/−/myo^+/+ (no. 441, 442, 443), and p53^+/+/myo^+/+ (no. 444) individuals (amplicon at 600 and 280 bp are for p53 and at 350 bp is for myo); (C) No significant (NS; Bonferroni's corrected P> 0.05) changes in HW:BW ratio among WT FVB, BL6 and crossbred FVB/BL6-WT mice in subsequent generations (F2 and F11 documented here), showing no changes in cardiac mass due to cross-breeding of two different parental backgrounds of the double-Tg mice. Results are presented as mean ± SEM and represent three independent experiments.