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. 2010 Mar 3;87(3):524–534. doi: 10.1093/cvr/cvq068

Figure 7.

Figure 7

Expression profiles of IPA™-predicted nodal molecules. (A) Immunoblot analysis showing downregulation of IPA-predicted nodal molecules Cdkn1a, Bcl2, c-Myc, and caspase (active caspase3) in p53−/−/myo+/+ mice, revealing active participation of these molecules in the prevention of HF in the absence of p53; (B) EMSA showing regression of NF-κB activity in p53−/−/myo+/+ mice; (C) Quantification of EMSA. Values obtained from three independent experiments are expressed as arbitrary units (AU), (Bonferroni's corrected P < 0.001). (D) Immunoblot analysis showing downregulation of IκB-α protein in p53−/−/myo+/+ mice correlating the regression of NF-κB activity in the absence of p53 to prevent HF. (GAPDH was analysed as a loading control for A & D).