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. 2010 Mar 7;87(3):440–448. doi: 10.1093/cvr/cvq077

Figure 3.

Figure 3

Interaction with PMCA maps to the region 735–934 of eNOS. (A) Schematic representation of Flag-tagged deletion mutants of eNOS. (B) Expression vectors encoding human PMCA2b and Flag-tagged deletion mutants of eNOS encoding amino acids 401–934, 193–735, or 1–505 were co-transfected in HEK 293 cells. Protein lysates were incubated with the 5F10 anti-PMCA monoclonal antibody. Immunoprecipitated proteins (upper panel) and the expression levels of Flag-tagged proteins prior to immunoprecipitation (lower panel) were probed by western blot with anti-Flag antibody. (C) Expression vectors encoding human PMCA2b and Flag-tagged deletion mutants encoding amino acids 735–934 or 1–505 were co-transfected in HEK 293 cells. Protein lysates were immunoprecipitated and probed by western blot as described in (B).