Table 1.
Primers pairs used by the KPC Resistance Assay Kit to obtain the specific blaKPC amplicons analysed by ESI-MSa
| Primer pair nameb | 4674 | 4675 | 4676 |
|---|---|---|---|
| forward (5′–3′) | TTGCTGGACACACCCATCCGTTAC | TACACCCGGACGCCTAACAAGGA | TGGAGCTGAACTCCGCCATCC |
| reverse (5′–3′) | TCTCCGCCACCGTCATGCCTG | TGCCCGTTGACGCCCAATCC | TCCAGTGCAGAGCCCAGTGTCAG |
| Expected base composition after ESI-MS analysisc | |||
| blaKPC-2 and all other blaKPC subtypes not listed below | A26 G26 C26 T19 | A22 G31 C30 T12 | A23 G28 C33 T16 |
| blaKPC-3 | A26 G26 C26 T19 | A22 G31 C29 T13 | A23 G28 C33 T16 |
| blaKPC-4 and blaKPC-5 | A26 G27 C25 T19 | A22 G31 C30 T12 | A23 G28 C33 T16 |
aSpecies identification of Enterobacteriaceae was obtained with the same kit using primers specific for the valS housekeeping gene: 358-forward, 5′-TCGTGGCGGCGTGGTTATCGA-3′; and 358-reverse, 5′-TCGGTACGAACTGGATGTCGCCGTT-3′.
bAll primer sequences for KPC detection are based on GeneBank ID EU784136.
cResults in bold indicate amplicons with different base compositions that identify different KPC variants.