Figure 1. Detection of MT1 and MT2 mutants by SDS-PAGE.
Lysates from HEK 293 cells transiently expressing the indicated receptors were separated by SDS-PAGE and analysis performed by Western blot using anti-Flag or anti-MT1 (MT1) (A) or anti-Myc or anti-MT2 antibodies (MT2) (B). Similar results were obtained in three additional experiments.